Summary
Type: SPE
Application #: 304750
Catalog#: 730952 - Chromab. Columns HR-XC, 3 ml, 200 mg
Detection:
Conditions: Column conditioning: 5 ml methanol, then 5 ml water
(Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum
Washing: With the following washing mixtures the impurities are removed:
a) 2 ml 0.1 N HCl
b) 2 ml methanol
Drying: With nitrogen or air
Elution: Analytes are eluted with 3 x 2 ml methanol / 10% NH3
Evaporation and reconstitution with 1 ml of mobile phase from subsequent HPLC
Authors: SPE department
Source: Macherey-Nagel